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  • Title: [Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin].
    Author: Xu YW, Qi Y, Han X, Xu LN, Xu QW, Peng JY.
    Journal: Zhongguo Zhong Yao Za Zhi; 2007 Dec; 32(24):2595-7. PubMed ID: 18338594.
    Abstract:
    OBJECTIVE: To develop a method for the preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography (HSCCC). METHOD: The crude alcohol extracts were eluted on a macroporous resin column and then purified by high speed counter-current chromatography (HSCCC). A two-phase solvent system composed of ethyl acetate: n-butanol: water (2 : 1 : 3) was used, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL x min(-1), while the apparatus rotated at 800 r x min(-1) and the eluate was detected at 254 nm. RESULT: 136 mg gentiopicrin with purity of 99.6% determined by HPLC were obtained from 300 mg crude extraction only in one-step separation and less than 200 minutes. CONCLUSION: The established method is simple, high efficiency and suitable for large-scale separation of gentiopicrin.
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