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  • Title: Immunohistochemical and in-situ hybridization study of hypoxia inducible factor-1alpha and angiopoietin-1 in a rabbit model of mandibular distraction osteogenesis.
    Author: He JF, Xie ZJ, Zhao H, Ge WL, Hu JA, Li YN, Gu ZY.
    Journal: Int J Oral Maxillofac Surg; 2008 Jun; 37(6):554-60. PubMed ID: 18339517.
    Abstract:
    The endogenous release of angiogenic factors in a rabbit mandibular distraction osteogenesis model was investigated. The spatial and temporal expression of hypoxia inducible factor-1alpha (HIF-1alpha) and angiopoietin-1 (Ang-1) was compared at different phases. The lengthened calluses were harvested on post-osteotomy days 13, 20, 34 and 48, and then stained with haematoxylin & eosin. Immunohistochemical (IHC) and in-situ hybridization (ISH) examination of HIF-1alpha and Ang-1 staining was performed. The ossification in the distracted gap was predominantly intramembranous and slightly endochondral. Expression of HIF-1alpha and Ang-1 was mainly detected in the cytoplasm of fibroblast-like cells, osteoblasts and immature osteocytes on day 13 and 20, but declined with bone maturation. HIF-1alpha was also detected in the nuclei of some osteoblasts. These results suggest that the production of HIF-1alpha and Ang-1 in the distracted gap may contribute to new bone formation during gradual distraction of the mandible.
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