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  • Title: Quantitative micro-Raman assessment of dentine demineralization, adhesive penetration, and degree of conversion of three dentine bonding systems.
    Author: Santini A, Miletic V.
    Journal: Eur J Oral Sci; 2008 Apr; 116(2):177-83. PubMed ID: 18353013.
    Abstract:
    Unreacted monomers in adhesive systems may cause a reduction in material properties, an increase in the long-term instability of the restoration, and pulpal irritation. The degree of dentine demineralization, adhesive penetration, and the degree of conversion (DC) across the dentine-adhesive interface of self-etch adhesives were measured using micro-Raman spectroscopy. Two-step, self-etch AdheSE, one-step self-etch AdheSE One, and etch-and-rinse Excite (control) (Ivoclar Vivadent AG, Schaan, Liechtenstein) were studied. Nine human molars were allocated to three groups and a flat dentine surface was prepared. A smear layer was produced by grinding dentine with 600-grit silicone-carbide discs under water. After application and polymerization of the adhesive, teeth were sectioned to produce four 1-mm-thick slices per tooth for micro-Raman spectroscopy. There were statistically significant differences in the depth of dentine demineralization between all adhesives. The depth and degree of demineralization decreased in the order: Excite>AdheSE>AdheSE One. The mean +/- standard deviation (SD) values for DC within the adhesive layer were 85.2 +/- 2.9% (Excite), 81.4 +/- 4.2% (AdheSE), and 54.3 +/- 10.1% (AdheSE One), and within the hybrid layer were 55.2 +/- 22.5% (Excite), 65.1 +/- 16.9% (AdheSE), and 42.0 +/- 16.2% (AdheSE One). All systems showed a discrepancy between dentine demineralization and adhesive penetration. A significant amount of unreacted monomers were associated with all systems but particularly with the etch-and-rinse system.
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