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  • Title: Synthesis of the NS 2 nonstructural protein messenger RNA of influenza A viruses occurs in the absence of viral protein synthesis.
    Author: Odagiri T, Tobita K, Tashiro M.
    Journal: Arch Virol; 1991; 120(3-4):281-8. PubMed ID: 1835573.
    Abstract:
    The NS 2 messenger RNA (mRNA) of several influenza A viruses was shown to be synthesized in primary transcription. Analysis of in vitro translation products of mRNAs from infected MDCK cells treated with cycloheximide indicated that the NS 2 mRNA in addition to the NS 1 mRNA was synthesized with PR/8, Udorn, and Aichi viruses. The findings indicated that the NS 1 mRNA of these viruses was able to be spliced into the NS 2 mRNA as a primary transcript without viral protein synthesis, although the extent of splicing varied among different virus strains.
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