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  • Title: Collagen IX in weight-bearing areas of human articular cartilage in late stages of osteoarthritis.
    Author: Koelling S, Kruegel J, Klinger M, Schultz W, Miosge N.
    Journal: Arch Orthop Trauma Surg; 2008 Dec; 128(12):1453-9. PubMed ID: 18357462.
    Abstract:
    AIM: To determine if the amounts of collagen IX mRNA and protein are higher in osteoarthritic cartilage from weight-bearing areas of the knee joint compared to non-weight bearing areas in patients with stage IV osteoarthritis (OA). METHODS: Normal and OA cartilage samples were obtained from 15 patients undergoing total knee replacement or necropsies. mRNA was measured by real time RT-PCR and proteins were detected by Western blot and localized at the light and ultrastructural level. RESULTS: Collagen IX was found throughout all cartilage layers in healthy and OA tissue. Cells deposited collagen IX in the pericellular and interterritorial matrix and a 66% higher amount of collagen IX was detected in the pericellular matrix of the weight-bearing areas adjacent to the main defect in comparison to the macroscopically intact areas. This is in line with a 3.72 times higher amount of the respective mRNA. CONCLUSION: The increased levels of collagen IX protein and its mRNAs found in the weight-bearing areas adjacent to the main cartilage defect might reflect an attempt on the part of the diseased cartilage tissue to stabilize and protect the remaining matrix of late-stage osteoarthritic cartilage from further destruction.
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