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  • Title: Increased VEGF165 expression in HCT116 colon cancer cells after transient transfection with a GFP vector encoding HIF-1 gene.
    Author: Lucarini G, Zizzi A, Belvederesi L, Kyriakidou K, Mazzucchelli R, Biagini G.
    Journal: J Exp Clin Cancer Res; 2007 Dec; 26(4):515-9. PubMed ID: 18365547.
    Abstract:
    Hypoxia occurs in most solid tumors as a result of inefficient vascular development and/or abnormal vascular architecture. During hypoxia, HIF-1alpha acts as the primary transcription factor functioning to activate multiple target genes, including vascular endothelial growth factor (VEGF). Several studies have demonstrated that in tumors HIF-1alpha mediates VEGF protein expression at the transcription level. We aimed to establish whether HCT116 colon cancer cell VEGF expression is regulated by HIF-1 levels after transient transfection with a GFP vector encoding the HIF-1alpha gene. HCT116 cell VEGF expression were therefore assayed by immunohistochemistry and ELISA. After transfection with phMGFP-HIF-1alpha, VEGF immunostaining was significantly increased in transfected cells as compared with untransfected HCT116 cells (p = 0.024, Student's t test); culture media VEGF levels assayed by ELISA were also significantly increased in transfected cells (p = 0.008, Student's t-test). These data suggest that HIF-1alpha may play an important role in colon cancer angiogenesis, both as a biomarker of metastatic potential and as a novel target for gene therapy.
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