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  • Title: Repetitive-sequence-based PCR using the DiversiLab system for identification of Aspergillus species.
    Author: Hansen D, Healy M, Reece K, Smith C, Woods GL.
    Journal: J Clin Microbiol; 2008 May; 46(5):1835-9. PubMed ID: 18367576.
    Abstract:
    Repetitive-sequence-based PCR (rep-PCR) using the DiversiLab system was investigated for identification of Aspergillus. Ninety-five clinical isolates, identified by conventional methods, and five ATCC strains were tested. Sequencing of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2) was performed on 2 isolates with discrepant rep-PCR and conventional results and 15 randomly selected outlier isolates. One isolate not identified by sequencing was excluded from analysis. After resolving discrepancies, all but one A. glaucus strain had >or=85% similarity to one or more strains of the same Aspergillus species in the mold database, thereby providing accurate identification. No isolate was misidentified.
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