These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Telomerase inhibition by synthetic nucleic acids and chemosensitization in human bladder cancer cell lines.
    Author: Kraemer K, Fuessel S, Meye A.
    Journal: Methods Mol Biol; 2007; 405():9-22. PubMed ID: 18369813.
    Abstract:
    The knockdown of genes that are over-expressed in cancer, and function in tumor onset and/or progression, is an attractive tool to impair the growth of tumor cells. Synthetic nucleic acids such as antisense oligodeoxynucleotides (AS-ODNs) or small-interfering RNAs (siRNAs) were applied against different tumor-associated transcripts, including the human telomerase reverse transcriptase (hTERT), to inhibit the proliferation of tumor cells and to sensitize them against chemotherapeutic (CT) agents. The efficacy of nucleic acid-based inhibitors was evaluated in vitro by determining the extent of down-regulation of the respective target mRNA and protein expression as well as by extensively investigating growth properties (e.g., viability, proliferation, apoptosis, and cell-cycle distribution) of the affected tumor cells. Methods for a successful down-regulation of hTERT and for the quantitative determination of resulting effects on cellular growth were described herein.
    [Abstract] [Full Text] [Related] [New Search]