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Title: Analysis of geminivirus DNA replication by 2-D gel. Author: Saunders K. Journal: Methods Mol Biol; 2008; 451():135-43. PubMed ID: 18370253. Abstract: The technique described was developed for the separation of begomovirus DNA. DNA products resulting from and during geminiviral replication are characterized by the application of strand-specific separation and identification by strand-specific DNA probing of Southern blots. The mapping of the initiation site of complementary-strand DNA synthesis, by this technique is also presented.[Abstract] [Full Text] [Related] [New Search]