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  • Title: Construction of infectious clones of double-stranded DNA viruses of plants using citrus yellow mosaic virus as an example.
    Author: Huang Q, Hartung JS.
    Journal: Methods Mol Biol; 2008; 451():525-33. PubMed ID: 18370278.
    Abstract:
    Double-stranded DNA (dsDNA) viruses of plants are believed to be plant pararetroviruses. Their genome is replicated by reverse transcription of a larger than unit-length terminally redundant RNA transcript of the viral genomic DNA using the virus-encoded replicase. In order to produce a cloned, infectious viral genome, the clone must be constructed in a binary vector and be longer than the full, unit-length viral genome. The clone can then be transferred by Agrobacterium-assisted inoculation into a suitable host plant to induce virus infection.
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