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  • Title: Hapten synthesis and monoclonal antibody-based immunoassay development for detection of the fungicide trifloxystrobin.
    Author: Mercader JV, Suárez-Pantaleón C, Agulló C, Abad-Somovilla A, Abad-Fuentes A.
    Journal: J Agric Food Chem; 2008 Apr 23; 56(8):2581-8. PubMed ID: 18373349.
    Abstract:
    High-affinity and selective monoclonal antibodies have been produced against the strobilurin fungicide trifloxystrobin. A battery of functionalized haptens has been synthesized, and conjugate-coated enzyme-linked immunosorbent assays following different procedures have been developed. On the one hand, a two-step conjugate-coated immunoassay was optimized using extended or short incubation times, with limits of detection of 0.10 ng/mL for the extended assay and 0.17 ng/mL for the rapid assay. On the other hand, an immunoassay in the conjugate-coated format was optimized following a procedure consisting of just one incubation step. This one-step assay had a limit of detection of 0.21 ng/mL. All of these assays showed detection limits for trifloxystrobin in the low parts per billion range, well below the common maximum residue limits for this pesticide in foodstuffs (50 microg/kg).
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