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  • Title: Novel quantitative electrophoretic analysis of endotoxins on microchips.
    Author: Kilár A, Farkas V, Kovács K, Kocsis B, Kilár F.
    Journal: Electrophoresis; 2008 Apr; 29(8):1713-22. PubMed ID: 18383013.
    Abstract:
    A novel microchip electrophoresis method was developed and applied for sensitive detection and quantitative analysis of endotoxins extracted from Gram-negative bacteria. The method provides a fast and quantitative differentiation of smooth and rough endotoxins based on the solubilization and complexation of the lipopolysaccharides with dodecylsulfate, and then with a fluorescent dye. The migration of the complexes was followed by LIF detection. The novel method is able to replace the SDS-PAGE with the advantage of high speed and better sensitivity, and by avoiding the laborious gel-preparation and silver staining.
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