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  • Title: A physiology study of Escherichia coli overexpressing phosphoenolpyruvate carboxykinase.
    Author: Kwon YD, Lee SY, Kim P.
    Journal: Biosci Biotechnol Biochem; 2008 Apr; 72(4):1138-41. PubMed ID: 18391462.
    Abstract:
    To determine whether intracellular ATP levels can be affected, Escherichia coli overexpressing phosphoenolpyruvate carboxykinase (pck) or phosphoenolpyruvate carboxylase (ppc) were grown in glucose minimal medium. The Pck-overexpressing cells showed approximately twice the intracellular ATP concentration, with 22% slower growth than the Ppc-overexpressing strain. This unexpected result of higher ATP coupled with slower growth is discussed based on transcriptome analysis.
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