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  • Title: Lipoprotein fractionation by a precipitation method. A simple quantitative procedure.
    Author: Onongbu IC, Lewis B.
    Journal: Clin Chim Acta; 1976 Sep 20; 71(3):397-402. PubMed ID: 183921.
    Abstract:
    A method is described for quantitation of the three major classes of serum lipoproteins. After precipitation of very low density lipoprotein (VLDL) using sodium dodecyl sulphate, the cholesterol and triglyceride content of this lipoprotein class is directly measured. In a second aliquot serum high density lipoprotein (HDL) lipids are measured after precipitation of VLDL and low density lipoprotein (LDL). LDL cholesterol and triglyceride contents are calculated by difference. The procedure requires 2 ml serum, and sensitivity is adequate to permit lipoprotein analyses on umbilical cord serum. Close agreement is observed between this precipitation method and preparative ultracentrifugation.
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