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  • Title: Transposon-based strategies for the identification of essential bacterial genes.
    Author: Reznikoff WS, Winterberg KM.
    Journal: Methods Mol Biol; 2008; 416():13-26. PubMed ID: 18392958.
    Abstract:
    We present a conceptual review of transposition-based strategies for determining gene essentiality on a one-by-one basis in bacteria. Many of the techniques are described in greater detail in individual chapters of this volume. The second section of this chapter deals with transposition-deletion-based strategies for determining the essentiality of blocks of genes. This latter approach has the potential to experimentally define the minimal required genome for a given organism.
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