These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Staining efficiency of specific proteins depends on the staining method: wheat gluten proteins.
    Author: van den Broeck HC, America AH, Smulders MJ, Gilissen LJ, van der Meer IM.
    Journal: Proteomics; 2008 May; 8(9):1880-4. PubMed ID: 18398878.
    Abstract:
    To analyze gluten proteins involved in celiac disease (CD) by proteomic analysis, prolamins extracted from hexaploid wheat varieties were analyzed by SDS-PAGE and 2-DE. Differences between staining methods (CBB, silver nitrate, SYPRO Ruby, and CyDye) were analyzed in comparison to immunoblotting. Staining efficiency varied per protein across methods, and complete staining of all gluten proteins could not be achieved by one of these methods. Care should be taken in the selection of staining method especially if one wants to relate the results to data obtained by immunoblotting.
    [Abstract] [Full Text] [Related] [New Search]