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  • Title: CD4+ T-cell activation by antigen-presenting cells infected with urease-deficient recombinant Mycobacterium bovis bacillus Calmette-Guérin.
    Author: Mukai T, Maeda Y, Tamura T, Miyamoto Y, Makino M.
    Journal: FEMS Immunol Med Microbiol; 2008 Jun; 53(1):96-106. PubMed ID: 18400013.
    Abstract:
    We constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG-Delta UT) that lacks urease, providing acidic intraphagosomal conditions to drive an effective human immune T-cell response. BCG-Delta UT-infected macrophages stimulated autologous CD4+ T cells more efficiently than parent BCG-infected macrophages. For further T-cell activation, BCG-Delta UT-infected macrophages required pretreatment with exogenous recombinant granulocyte-macrophage colony-stimulating factor or costimulation with either CD40 ligand or interferon-gamma. By contrast, BCG-Delta UT-infected dendritic cells induced significant activation of naïve CD4+ T cells without costimulating signals. C57BL/6 mice intradermally inoculated with BCG-Delta UT more efficiently produced memory T cells that responded to recall antigen. Therefore, the depletion of urease from BCG is useful for the activation of T cells.
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