These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Heat stress causes aberrant DNA methylation of H19 and Igf-2r in mouse blastocysts.
    Author: Zhu JQ, Liu JH, Liang XW, Xu BZ, Hou Y, Zhao XX, Sun QY.
    Journal: Mol Cells; 2008 Apr 30; 25(2):211-5. PubMed ID: 18413996.
    Abstract:
    To gain a better understanding of the methylation imprinting changes associated with heat stress in early development, we used bisulfite sequencing and bisulfite restriction analysis to examine the DNA methylation status of imprinted genes in early embryos (blastocysts). The paternal imprinted genes, H19 and Igf-2r, had lower methylation levels in heat-stressed embryos than in control embryos, whereas the maternal imprinted genes, Peg3 and Peg1, had similar methylation pattern in heat-stressed embryos and in control embryos. Our results indicate that heat stress may induce aberrant methylation imprinting, which results in developmental failure of mouse embryos, and that the effects of heat shock on methylation imprinting may be gene-specific.
    [Abstract] [Full Text] [Related] [New Search]