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  • Title: [Development and application of a two-step MacELISA for the early diagnosis of hemorrhagic fever with renal syndrome].
    Author: Zhang QF, Li JD, Li WH, Li C, Liu QZ, Liang MF, Li DX.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2008 Feb; 22(1):6-8. PubMed ID: 18414686.
    Abstract:
    OBJECTIVE: To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure. METHODS: The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA. RESULTS: This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%. CONCLUSION: This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.
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