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Title: [Epidemiological survey on the infection of hepatitis E virus among pigs in Henan province]. Author: Li XJ, Zhao CY, Fan JP, Song AJ, Wang YC, Zhang JG. Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2008 Feb; 22(1):24-6. PubMed ID: 18414692. Abstract: OBJECTIVE: To investigate hepatitis E virus (HEV) infection among pigs in Henan province. METHODS: A total of 623 swine sera, collected from 5 districts, were divided into two groups, under 3-month of age and over 3-month of age. They were tested for HEV antigen and antibody by using ELISAs, respectively. The sera positive for HEV antigen were tested for HEV RNA with RT-PCR. The positive products of RT-PCR were cloned and sequenced. RESULTS: The positive rates of anti-HEV antibody of the groups under 3-month and over 3-month of age were 90.27% and 92.55%, respectively, without statistical difference, while those of HEV antigen were 15.93% and 5.69%, respectively, with significant difference. The positive rates of anti-HEV antibody and HEV antigen were significantly different among different districts. HEV RNA was detectable in 5 of 47 HEV antigen positive samples. The sequence analysis showed that in 4 of 5 specimens the sequence belonged to genotype 4 while in the remaining one the sequence was genotype 1. CONCLUSION: The prevalence rate of HEV infection in pigs was high in Henan province and the rate differed in different districts.[Abstract] [Full Text] [Related] [New Search]