These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Prokaryotic expression, purification of prM of JEV and preparation of monoclonal antibody].
    Author: Ning BF, Zhu HM, Zhou XJ, Cao Y, Zhou AG.
    Journal: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2008 Feb; 22(1):65-7. PubMed ID: 18414706.
    Abstract:
    OBJECTIVE: To prepare monoclonal antibody (mAb) against prM epitope. METHODS: The gene encoding prM was isolated using RT-PCR from brain of JEV infected mouse and cloned into prokaryotic expression vector pET-32a. Recombinant plasmid was transformed into E.coli BL21/DE3/LysS, then the transformed cells were expressed with the induction of IPTG. The expression and purification of the prM protein was analyzed by SDS-PAGE. The BALB/c mice were immunized with purified prM protein. Hybridoma cell lines secreting monoclonal antibodies against prM were established after cell fusion of mouse splenic cell and P3-X63-Ag8.653 cells. The specificity of mAb was identified by ELISA, Western Blot and Immunohistochemistry assay. RESULTS: mAb against prM epitope of JEV was prepared successfully. CONCLUSION: The obtained prM specific mAb was valuable for the prevention and dignosis of Japanese encephalitis.
    [Abstract] [Full Text] [Related] [New Search]