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  • Title: Isolation and partial characterization of circulating immune complexes in sera of children with HBV-mediated glomerulonephritis.
    Author: Gregorek H, Jung A, Grabowska B, Madaliński K.
    Journal: Arch Immunol Ther Exp (Warsz); 1991; 39(5-6):519-27. PubMed ID: 1841549.
    Abstract:
    Circulating immune complexes (CIC) containing HBsAg and HBeAg were identified in sera of 5 out of 6 children with hepatitis B mediated membranous glomerulonephritis. CIC were precipitated from sera by 3.5% PEG, washed and subsequently analysed after acid dissociation and trypsin digestion. HBsAg, anti-HBs and albumin; HBeAg, anti-HBe and anti-HBc were recovered from the isolated complexes and these findings are discussed. Analysis of 3.5% PEG mediated precipitate of human serum proteins showed the relatively high content of IgG classical pathway complement components: C1q, C4 and C3.
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