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  • Title: Mutant strains of Streptomyces cinnamonensis protoplasts. Cultural and physiological conditions.
    Author: Matĕjů J, Marsálková J, Nohýnek M, Steinerová N.
    Journal: Folia Microbiol (Praha); 1991; 36(1):42-8. PubMed ID: 1841848.
    Abstract:
    Mycelium of Streptomyces cinnamonensis mutant strains cultivated in a synthetic medium with glycine produced protoplasts after lysis of cell walls with lysozyme. The protoplast yield was up to 95%. The protoplasts could revert and mycelial forms were thus regenerated. In a sucrose-containing medium the protoplasts stored at 4 degrees C were stable for 2 d.
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