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  • Title: The effect of anticoagulant, storage temperature and dilution on cord blood hematology parameters over time.
    Author: Freise KJ, Schmidt RL, Gingerich EL, Veng-Pedersen P, Widness JA.
    Journal: Int J Lab Hematol; 2009 Oct; 31(5):496-504. PubMed ID: 18422712.
    Abstract:
    The objective of the study was to determine whether selected hematologic parameters measured on umbilical cord blood samples using an automated hematology analyzer (Sysmex XE-2100) were affected by (i) anticoagulant (the specimens were collected in EDTA vs. sodium heparin), (ii) temperature (the specimens were maintained at 4 degrees C vs. room temperature for up to 72 h) and (iii) 1 : 5 dilution vs. undiluted using the manufacturer's diluting solution. Use of heparin, instead of EDTA, had little effect on the hematologic results (n = 8) except for lower platelet and progenitor cell counts. Results were remarkably stable for 72 h at either room temperature or 4 degrees C except for modest red blood cell swelling at 24 h. Specimens of blood diluted at 1 : 5 had an immediate small, but significant change on white cell count (+13.3%), reticulocyte count (-11.2%) and reticulocyte hemoglobin content (-19.6%). Diluted samples did not change further over 4 h at room temperature. With a 1 : 5 dilution, analysis of 40 microl of cord blood stored for 3 days at room temperature may provide useful hematologic information with little phlebotomy loss.
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