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  • Title: Slot-blot analysis of 3-nitrotyrosine-modified brain proteins.
    Author: Sultana R, Butterfield DA.
    Journal: Methods Enzymol; 2008; 440():309-16. PubMed ID: 18423227.
    Abstract:
    3-Nitrotyrosine (3-NT) is used as a biomarker of nitrosative stress. The formation of 3-NT has been reported in a number of diseases, including Alzheimer's disease, Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, and cancer. The nitration of proteins is a reversible process, but it can induce a conformational change and thereby functional alterations of the affected protein. 3-NT measurements in biological samples are usually carried out by methodologies such as immunohistochemistry, high-performance liquid chromatography, gas chromatography, and immunochemical detection. This chapter describes the immunochemical method for the determination of protein-bound 3-NT using slot-blot analysis.
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