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  • Title: Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry.
    Author: Ortner K, Buchberger W.
    Journal: Electrophoresis; 2008 May; 29(10):2233-7. PubMed ID: 18425754.
    Abstract:
    A simple method for the determination of the two most abundant sialic acids released from glycoproteins based on CZE-MS is presented. Several parameters like BGE with various organic modifiers and sheath liquids were studied with respect to their suitability for the fast and easy analysis of the selected compounds by CZE-MS. Finally, a BGE containing 10 mM ammonium acetate allowed the quantification of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) in glycoproteins as well as human plasma samples. LOD and LOQ were 2 microg/mL and 6 microg/mL, respectively.
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