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  • Title: Detection of mutations by denaturing gradient gel electrophoresis.
    Author: Børresen-Dale AL, Hovig E, Smith-Sørensen B.
    Journal: Curr Protoc Hum Genet; 2001 May; Chapter 7():Unit 7.5. PubMed ID: 18428306.
    Abstract:
    This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels.
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