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  • Title: Selective isolation of mammalian genes by TAR cloning.
    Author: Kouprina N, Larionov V.
    Journal: Curr Protoc Hum Genet; 2006 May; Chapter 5():Unit 5.17. PubMed ID: 18428393.
    Abstract:
    Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.
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