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  • Title: Size-exclusion chromatography with on-line light scattering.
    Author: Arakawa T, Wen J.
    Journal: Curr Protoc Protein Sci; 2001 Nov; Chapter 20():Unit 20.6. PubMed ID: 18429161.
    Abstract:
    This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight of glycoproteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.
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