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  • Title: Purification of immunoglobulin G.
    Author: Andrew SM, Titus JA.
    Journal: Curr Protoc Immunol; 2001 May; Chapter 2():Unit 2.7. PubMed ID: 18432771.
    Abstract:
    IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.
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