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  • Title: Detection of adenosine using surface-enhanced Raman scattering based on structure-switching signaling aptamer.
    Author: Chen JW, Liu XP, Feng KJ, Liang Y, Jiang JH, Shen GL, Yu RQ.
    Journal: Biosens Bioelectron; 2008 Sep 15; 24(1):66-71. PubMed ID: 18436440.
    Abstract:
    In the present study, we report a novel sensitive method for the detection of adenosine using surface-enhanced Raman scattering (SERS) sensing platform based on a structure-switching aptamer. First, Ag-clad Au colloids film on a polished gold disc is prepared as enhanced substrate and modified with thiolated capture DNA. The formation of an aptamer/DNA duplex of expanded anti-adenosine aptamer and tetramethylrhodamine-labeled DNA (denoted TMR-DNA) is then developed, in which TMR-DNA could also hybridize completely with capture DNA. The introduction of adenosine thus triggers structure switching of the aptamer from aptamer/DNA duplex to aptamer/target complex. As a result, the released TMR-DNA is captured onto the SERS substrate, resulting in an increase of SERS signal. Under optimized assay conditions, a wide linear dynamic range (2.0 x 10(-8)M to 2 x 10(-6)M) was reached with low detection limit (1.0 x 10(-8)M). Moreover, high selectivity, stability and facile regeneration are achieved. The successful test demonstrates the feasibility of the strategy for adenosine assay.
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