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  • Title: In vitro development and pregnancy outcomes for human embryos cultured in either a single medium or in a sequential media system.
    Author: Sepúlveda S, Garcia J, Arriaga E, Diaz J, Noriega-Portella L, Noriega-Hoces L.
    Journal: Fertil Steril; 2009 May; 91(5):1765-70. PubMed ID: 18439587.
    Abstract:
    OBJECTIVE: To compare a single medium, with medium renewal on day 3, with a sequential media system for development of human embryos to the blastocyst stage and subsequent pregnancy outcome. DESIGN: Prospectively randomized study using donor oocytes. SETTING: Private infertility clinic. PATIENT(S): Oocytes were collected from 47 donors (mean +/- SD age = 25.6 +/- 3.5 years) from March to October 2006 and used for 79 patients (40.6 +/- 5.4 years). The patients were prospectively randomized to have their embryos cultured in a single medium or in a sequential media system. INTERVENTION(S): The donor oocytes were fertilized with fresh patient partner sperm and the resultant zygotes cultured in either a single medium or a sequential media system from day 1 until the blastocyst stage. After endometrial preparation the embryos were transferred to the patients on day 5 or day 6. MAIN OUTCOME MEASURE(S): In vitro embryo developmental characteristics and pregnancy and implantation rates. RESULT(S): In vitro development rates on days 3, 4, and 5 and implantation rates were significantly greater for embryos cultured in the single medium than for those cultured in the sequential media system. CONCLUSION(S): A single medium was as good as or better than a sequential media system for human embryo culture from the zygote to blastocyst stage. The idea that a sequential media system is required for optimal development of the human embryo to the blastocyst stage is highly questionable.
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