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  • Title: Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients.
    Author: Cohen-Bacrie P, Belloc S, Ménézo YJ, Clement P, Hamidi J, Benkhalifa M.
    Journal: Fertil Steril; 2009 May; 91(5):1801-5. PubMed ID: 18439595.
    Abstract:
    OBJECTIVE: To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation. DESIGN: Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study. SETTING: All the patients were under clinical management, consulting for hypofertility at a fertility center in France. PATIENT(S): One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis. INTERVENTION(S): Sperm analysis and DNA damage testing. MAIN OUTCOME MEASURE(S): Sperm morphology, DNA fragmentation, and movement characteristics. RESULT(S): One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results. CONCLUSION(S): World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures.
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