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  • Title: Enzymatic activity of immobilized enzyme determined by isothermal titration calorimetry.
    Author: Henzler K, Haupt B, Ballauff M.
    Journal: Anal Biochem; 2008 Jul 15; 378(2):184-9. PubMed ID: 18440294.
    Abstract:
    The activity of adsorbed beta-glucosidase onto spherical polyelectrolyte brushes (SPBs) is investigated by UV-Vis spectroscopy and isothermal titration calorimetry (ITC). By comparing the results of these two methods, we demonstrate that ITC is a precise method for the study of the activity of immobilized enzymes. The carrier particles used for immobilization here consist of a polystyrene core onto which poly(acrylic acid) chains are grafted. High amounts of enzyme can be immobilized in the brush layer at low ionic strength by the polyelectrolyte-mediated protein adsorption (PMPA). Analysis of the activity of beta-glucosidase was done in terms of Michaelis-Menten kinetics. Moreover, the enzymatic activity of immobilized enzyme is studied by ITC using cellobiose as substrate. All data show that ITC is a general method for the study of the activity of immobilized enzymes.
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