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  • Title: Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow-based P-selectin-coated microtube.
    Author: Narasipura SD, Wojciechowski JC, Duffy BM, L Liesveld J, King MR.
    Journal: Am J Hematol; 2008 Aug; 83(8):627-9. PubMed ID: 18442063.
    Abstract:
    Recently, a flow-based selectin-dependent method for capture and enrichment of specific type blood cells (CD34+ hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD45+ blood cells directly from human bone marrow to a high purity (90%-97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD45+ cell enrichment procedures required for chimerism analysis following allogenic stem-cell transplantation.
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