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  • Title: Fingerprint of Hedyotis diffusa Willd. by HPLC-MS.
    Author: Yang T, Yang YH, Yang JY, Chen BM, Duan JP, Yu SY, Ouyang HT, Cheng JP, Chen YX.
    Journal: Phytochem Anal; 2008; 19(6):487-92. PubMed ID: 18446772.
    Abstract:
    A HPLC-MS fingerprint method has been developed based on the consistent chromatographic features of the major chemical constituents among 10 batches of Hedyotis diffusa Willd. Chromatographic separation was conducted on a Hypersil-Keystone Hypurity C(18) column using methanol:water:acetic acid as the mobile phase. Major compounds, including oleanolic acid, ursolic acid and ferulic acid, were analysed by HPLC-MS. Their analysis was ascertained by comparison with data derived from the standard compounds. The HPLC-MS fingerprint was successfully applied to analyse and differentiate samples from different geographical origins, or processing methods. H. diffusa was well distinguished from Hedyotis chrysotricha by HPLC-MS. Therefore the establishment of fingerprint of H. diffusa is critical in assessing and controlling its overall quality.
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