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  • Title: [The production and analysis of human anti-T-lymphocyte-receptor serum heterologous for sheep erythrocytes].
    Author: Itano EN, Koga LV, Ono MA, Tonon J, Ferreira CS, Sumigawa M, Kaminami H.
    Journal: Semina; 1991 Jun; 12(2):70-5. PubMed ID: 1845308.
    Abstract:
    Anti-human T lymphocyte serum specific to the receptor for sheep erythrocytes (E) was produced by immunizing sheep with autologous erythrocytes sensitized with solubilized receptors from human lymphocytes. The anti-soluble receptors serum (anti-Rs) inhibited E-Rosette formation, identified T lymphocytes by indirect immunofluorescence, agglutinated the formalized E-soluble receptors complexes, and inhibited the E-soluble receptors interaction. This anti-Rs serum was also submitted to the affinity chromatography by protein A sepharose, and the amounts of 0.15 to 120 micrograms of purified anti-Rs IgG were added to the lymphocyte culture. The result obtained showed an induction of proliferative response of the normal human lymphocytes in the presence of 60 and 120 micrograms of anti-Rs IgG.
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