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  • Title: Fabrication and application of single nucleotide polymorphisms library on magnetic nanoparticles using adaptor PCR.
    Author: Liu H, Li S, Ji M, Nie L, Chen J, Miao Y, He N.
    Journal: J Nanosci Nanotechnol; 2008 Jan; 8(1):405-9. PubMed ID: 18468091.
    Abstract:
    We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNP. Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.
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