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  • Title: Liver-directed gene therapy using the sleeping beauty transposon system.
    Author: Belur LR, McIvor RS, Wilber A.
    Journal: Methods Mol Biol; 2008; 434():267-76. PubMed ID: 18470650.
    Abstract:
    Sleeping Beauty (SB) is a transposon system genetically reconstructed from teleost fish that mediates chromosomal integration of DNA sequences by a cut-and-paste mechanism. SB has been shown to mediate transposition in a variety of cells and tissues, has been used for the generation of transgenic animals and has been tested as a vector for gene therapy in several animal models of human disease. Here, we describe methods that we have developed for testing SB-mediated transposition, first in cultured mammalian cells, and then in vivo using a combination of rapid, high-volume tail vein injection for DNA delivery to the liver along with in vivo bioluminescence imaging to monitor sustained luciferase gene expression in individual animals.
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