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  • Title: Rapid purification of protein phosphatase 2A from mouse brain by microcystin-affinity chromatography.
    Author: Nishiwaki S, Fujiki H, Suganuma M, Nishiwaki-Matsushima R, Sugimura T.
    Journal: FEBS Lett; 1991 Feb 11; 279(1):115-8. PubMed ID: 1847341.
    Abstract:
    Microcystin LR, which is a monocyclic heptapeptide containing two L-amino acids, leucine and arginine, is a new inhibitor of protein phosphatases 1 and 2A. Microcystin LR-affinity chromatography was used to purify protein phosphatase 2A as a holoenzyme. Five mg of microcystin LR were immobilized to ECH Sepharose 4B by the carbodiimide coupling reaction. Following DEAE-cellulose column chromatography, microcystin-affinity chromatography, as the second step in the procedure, resulted in purification of protein phosphatase 2A in a pure form. The enzyme isolated from mouse brain consisted of two regulatory subunits of 67 kDa and 58 kDa and a catalytic subunit of 41 kDa. Microcystin-affinity chromatography is useful for isolation of protein phosphatase 2A.
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