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  • Title: A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples.
    Author: Lee HY, Kim NY, Park MJ, Yang WI, Shin KJ.
    Journal: Biotechniques; 2008 Apr; 44(4):555-6, 558. PubMed ID: 18476821.
    Abstract:
    To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.
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