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Pubmed for Handhelds

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  • Title: A novel assay reveals the blockade of esterases by piperonyl butoxide.
    Author: Khot AC, Bingham G, Field LM, Moores GD.
    Journal: Pest Manag Sci; 2008 Nov; 64(11):1139-42. PubMed ID: 18481337.
    Abstract:
    BACKGROUND: Conventional in vitro assays sometimes fail to reveal esterase inhibition by piperonyl butoxide (PBO), although synergism studies suggest loss of esterase-mediated sequestration of insecticide does take place. A new in vitro assay has been devised that routinely reveals binding between PBO and these esterases. RESULTS: The new 'esterase interference' assay detects the blockade of resistance-associated esterases in a species, Myzus persicae Sülzer, where this has not previously been seen. The assay also demonstrates directly the protective effect esterases may confer to target sites of insecticides. CONCLUSION: The new assay reveals esterase blockade by PBO and thus has the potential to be used as a high-throughput screening method for other potential synergists.
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