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  • Title: Expression of insulin-like growth factor-IA and factor-IB mRNA in human liver, hepatoma cells, macrophage-like cells and fibroblasts.
    Author: Nagaoka I, Someya A, Iwabuchi K, Yamashita T.
    Journal: FEBS Lett; 1991 Mar 11; 280(1):79-83. PubMed ID: 1849099.
    Abstract:
    The human insulin-like growth factor-I (IGF-I) gene codes for two transcripts, IGF-IA and IGF-IB mRNAs, formed by alternative splicing. In this study, the expression of these IGF-I mRNA transcripts was examined using human liver, hepatoma cells, macrophage-like cells and fibroblasts. The reverse transcription-polymerase chain reaction revealed that these cells contained both IGF-IA mRNA (representing exons I, II, III and V) and IGF-IB mRNA (representing exons I, II, III and IV). Interestingly, an RNase protection assay using 32P-labeled IGF-IA and IGF-IB exon-specific cRNA probes demonstrated that IGF-IA mRNA was 10-fold more abundant than IGF-IB mRNA in these cells. However, there was no difference in the stabilities of IGF-IA and IGF-IB mRNAs. These observations indicate that IGF-IA mRNA is more expressed than IGF-IB mRNA in these cells independent of their stabilities.
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