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  • Title: Is lack of susceptible recipients in the intestinal environment the limiting factor for transduction of Shiga toxin-encoding phages?
    Author: Sekse C, Solheim H, Urdahl AM, Wasteson Y.
    Journal: J Appl Microbiol; 2008 Oct; 105(4):1114-20. PubMed ID: 18492045.
    Abstract:
    AIM: To determine whether a Shiga toxin 2 (Stx2)-encoding phage from Escherichia coli O157:H7 could be transmitted to commensal E. coli in a ruminant host without adding a specific recipient strain. METHODS AND RESULTS: Sheep were inoculated with an E. coli O157:H7 strain containing an Stx2-encoding bacteriophage (Phi3538) in which a chloramphenicol-resistant gene, cat, is inserted into stx(2). A total of 149 faecal samples were sampled and analysed for detection and quantification of E. coli O157:H7 and presumptive transductants. Phage Phi3538 (Deltastx(2)::cat) was demonstrated to be transduced to an ovine E. coli O175:H16 at one occasion. CONCLUSIONS: The study demonstrates an in vivo transduction in sheep from an E. coli O157:H7 strain to an ovine E. coli O175:H16. A functional Stx2-encoding phage was incorporated into the host's DNA. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first in vivo stx phage transduction study reported in which a recipient strain was not fed to the test animals. We suggest that the access to susceptible hosts is one main limiting factor for transduction to occur in the intestine.
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