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  • Title: Evaluation of poly(ADP-ribose) polymerase cleavage (cPARP) in ejaculated human sperm fractions after induction of apoptosis.
    Author: Mahfouz RZ, Sharma RK, Poenicke K, Jha R, Paasch U, Grunewald S, Agarwal A.
    Journal: Fertil Steril; 2009 May; 91(5 Suppl):2210-20. PubMed ID: 18501896.
    Abstract:
    OBJECTIVE: To examine the presence of cleaved poly(ADP-ribose) polymerase(s) (cPARP) in ejaculated spermatozoa and determine cPARP levels following exposure to chemical or oxidative stress. DESIGN: Prospective pilot study. SETTING: Tertiary care academic hospital. PATIENT(S): Eight healthy men. INTERVENTION(S): Semen specimens were collected, prepared with double-density-gradient centrifugation, and divided into control, hydrogen peroxide (H(2)O(2)), H(2)O(2) + 3-aminobenzamide (3-ABA), staurosporine (STS), and STS + 3-ABA treated groups. MAIN OUTCOME MEASURE(S): Cleaved PARP and apoptosis markers by flow cytometry. RESULT(S): Cleaved PARP was detected in both neat and mature fractions. The cPARP levels were similar in both mature and immature spermatozoa. In combined mature and immature fractions, a higher percentage of late apoptotic sperm was seen in STS + 3-ABA versus STS. Higher levels of late apoptotic spermatozoa were seen in immature versus mature fractions within STS and STS + 3-ABA groups. Lower levels of cPARP were seen in immature versus mature fractions in H(2)O(2) and H(2)O(2) + 3-ABA treated groups. Cleaved PARP was related to activated caspase-3. CONCLUSION(S): Cleaved PARP is present in ejaculated human spermatozoa. Poly(ADP-ribose) polymerase inhibitors may play a different role in chemical versus oxidative stress-induced sperm damage.
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