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  • Title: Downregulation of IL-13 gene transcription by T-bet in human T cells.
    Author: Suzuki K, Kaminuma O, Hiroi T, Kitamura F, Miyatake S, Takaiwa F, Tatsumi H, Nemoto S, Kitamura N, Mori A.
    Journal: Int Arch Allergy Immunol; 2008; 146 Suppl 1():33-5. PubMed ID: 18504404.
    Abstract:
    BACKGROUND: Downregulation of a Th2 cytokine, IL-4, by a Th1-specific transcription factor, T-bet, has been demonstrated. However, the regulatory role of T-bet in another Th2 cytokine, IL-13, is not fully delineated. METHODS: IL-13 mRNA expression in Jurkat cells was examined by quantitative RT-PCR, while the transcriptional activity of 5'-flanking region in the IL-13 gene encompassing -1077 to +49 was investigated by fluorescence-based promoter reporter assay. The effect of T-bet was investigated by transfection of the cells with the T-bet expression vector. RESULTS: Stimulation with phorbol ester plus Ca2+ ionophore clearly induced IL-13 gene transcription in Jurkat cells. Ectopically expressed T-bet significantly suppressed the inducible mRNA expression and promoter activity of IL-13. CONCLUSION: IL-13 expression was downregulated by T-bet at the level of gene transcription, independently of the modulation of Th1/Th2 balance. T-bet is the potential key factor in the development of Th1/Th2-related diseases.
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