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  • Title: Use of the polymerase chain reaction to detect herpes simplex virus DNA in paraffin sections of human brain at necropsy.
    Author: Nicoll JA, Maitland NJ, Love S.
    Journal: J Neurol Neurosurg Psychiatry; 1991 Feb; 54(2):167-8. PubMed ID: 1850452.
    Abstract:
    The feasibility of detecting herpesvirus DNA in paraffin sections of routinely fixed and processed human necropsy brains by use of the polymerase chain reaction (PCR) was assessed. A 110 bp segment of the thymidine kinase gene of herpes simplex virus type 1 (HSV1) could readily be amplified in sections from the brains of six patients with acute HSV1 encephalitis but not from those of six patients with other neurological diseases, including varicella-zoster encephalitis and herpes simplex virus type 2 encephalitis. Primers suitable for amplifying c-myc were included in the PCRs for assessment of DNA preservation. This was found to be adequate to allow amplification of c-myc DNA in sections from all of the brains studied. The PCR provides a simple and specific means of detecting HSV1 DNA in routinely processed necropsy material.
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