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  • Title: [Polymerase chain reaction to determine and control drug-resistant Mycobacterium tuberculosis strains].
    Author: Vladimirskiĭ MA, Aliapkina IuS, Varlamov DA, Alekseev IaI, Shipina LK, Shul'gina MV, Domotenko LV, Bykadorova KR, Gashchenko NN, Endourova LB, Ivanova OV, Il'ina EA, Levkova OA, Markova TV, Nazemtseva VP, Pavlova EP, Polozov AI, Shishkina NV.
    Journal: Probl Tuberk Bolezn Legk; 2008; (4):38-44. PubMed ID: 18509912.
    Abstract:
    Real-time polymerase chain reaction was used to develop a one-stage procedure for molecular genetic analysis of Mycobacterium tuberculosis (MBT) DNA in order to determine mutations associated with drug resistance to the antituberculous agents: isoniazid and rifampicin. To analyze the spread of drug-resistance of the causative agent of tuberculosis in Russia, two thousand MBT strains were studied in 24 regions of all the federal districts. Testing 1406 MBT strains isolated by first detected and untreated patients revealed multidrug resistance (MDR) in 21.9% of cases. MRD was detected in 58.5% of the previously treated patients with MDR. The agreement of molecular genetic analysis of drug resistance with the results of cultural tests of 1096 strains was 94%.
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