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  • Title: Simultaneous detection and deconvolution of congested NMR spectra containing three isotopically labeled species.
    Author: Masterson LR, Tonelli M, Markley JL, Veglia G.
    Journal: J Am Chem Soc; 2008 Jun 25; 130(25):7818-9. PubMed ID: 18512910.
    Abstract:
    We present a procedure for isolating subspectra corresponding to individual protein or peptide components in a ternary mixture or complex. Each of the three-component species is labeled differently: species A uniformly with 15N, species B uniformly with 15N and 13C, and species C uniformly with 15N but selectively with 13C' or 13Calpha. By using the dual carbon label selective HSQC (DCLS-HSQC) pulse sequence and exploiting differences in 1J 15N-13C coupling patterns to filter selected 15N resonances from detection during a constant time period, a subspectrum from each species can be generated from three spectra acquired from a single sample. Many important biological pathways involve dynamic interactions among members of multicomponent protein assemblies, and this approach offers a powerful way to monitor such processes.
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