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  • Title: Indirect immunofluorescence on HEp-2000 substrate is a sensitive method for detecting anti-Ro/SSA antibodies in patients with lupus erythematosus and/or photosensitivity.
    Author: Cozzani E, Drosera M, Siccardi M, Babbini G, Parodi A.
    Journal: J Rheumatol; 2008 Jul; 35(7):1320-2. PubMed ID: 18528967.
    Abstract:
    OBJECTIVE: To evaluate the sensitivity of indirect immunofluorescence (IIF) utilizing HEp-2000 cells to detect antinuclear and anti-Ro/SSA antibodies. METHODS: We examined 5949 sera samples from 2316 patients with connective tissue diseases. All sera were tested by IIF on HEp-2 cells as substrate and by counterimmunoelectrophoresis (CIE). The positive sera for anti-Ro/SSA antibodies by CIE were retested by IIF using HEp-2000 cells. The sera that were anti-Ro/SSA-positive in CIE but negative on HEp-2000 cells were tested by ELISA for the 60 and 52 subunits of Ro/SSA antigen. RESULTS: Our results confirm the high sensitivity (81%) of HEp-2000 cells in detecting anti Ro/SSA antibodies. CONCLUSION: Using the HEp-2000 substrate is a reliable, simple alternative method for detecting anti-Ro/SSA antibodies in a first-level screening test such as IIF, especially in patients with lupus erythematosus and/or photosensitivity.
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